WB analysis of whole cell extracts (30 microg lysate) of A549 (Lane 1), Hep G2 (lane 2) and PANC-1 (Lane 3) using GTX54748 eIF1 antibody [2B9]. Dilution : 1:500-1:2000
![WB analysis of 80microg whole cell lysate using GTX54748 eIF1 antibody [2B9]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX54748/GTX54748_2129_WB_w_23060900_116.webp "WB analysis of 80microg whole cell lysate using GTX54748 eIF1 antibody [2B9]. Dilution : 1:1000")
![ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton? X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX54748/GTX54748_800_ICC-IF_w_23060900_545.webp "ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton? X-100 for 10 minutes Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of HeLa cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX54748/GTX54748_801_ICC-IF_w_23060900_785.webp "ICC/IF analysis of HeLa cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC")
![ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX54748/GTX54748_802_ICC-IF_w_23060900_173.webp "ICC/IF analysis of MCF-7 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC")
![ICC/IF analysis of NIH-3T3 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX54748/GTX54748_803_ICC-IF_w_23060900_309.webp "ICC/IF analysis of NIH-3T3 cells using GTX54748 eIF1 antibody [2B9]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 incubated overnight at 4 oC")
![ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX54748 eIF1 antibody [2B9]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX54748/GTX54748_804_ICC-IF_w_23060900_877.webp "ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX54748 eIF1 antibody [2B9]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
WB analysis of whole cell extracts (30 microg lysate) of A549 (Lane 1), Hep G2 (lane 2) and PANC-1 (Lane 3) using GTX54748 eIF1 antibody [2B9]. Dilution : 1:500-1:2000
eIF1 antibody [2B9]
GTX54748
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEIF1
Overview
- SupplierGeneTex
- Product NameeIF1 antibody [2B9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:2000. ICC/IF: 1-2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2B9
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID10209
- Target nameEIF1
- Target descriptioneukaryotic translation initiation factor 1
- Target synonymsA121; EIF-1; EIF1A; eukaryotic translation initiation factor 1; ISO1; protein translation factor SUI1 homolog; SUI1; sui1iso1
- HostMouse
- IsotypeIgG2a
- Protein IDP41567
- Protein NameEukaryotic translation initiation factor 1
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- DHX29 reduces leaky scanning through an upstream AUG codon regardless of its nucleotide context. Pisareva VP et al., 2016 May 19, Nucleic Acids ResRead more