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Immunofluorescence staining of A549 cells with CSB-PA007503LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cells with CSB-PA007503LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cells with CSB-PA007503LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EIF1 Antibody

CSB-PA007503LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetEIF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EIF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10209
  • Target name
    EIF1
  • Target description
    eukaryotic translation initiation factor 1
  • Target synonyms
    A121, EIF-1, EIF1A, ISO1, SUI1, eukaryotic translation initiation factor 1, protein translation factor SUI1 homolog, sui1iso1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41567
  • Protein Name
    Eukaryotic translation initiation factor 1
  • Scientific Description
    Necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EIF1 using anti-EIF1 antibody (A04125-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 7: rat pancreas tissue lysates, Lane 8: mouse pancreas tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF1 antigen affinity purified polyclonal antibody (Catalog # A04125-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF1 at approximately 13 kDa. The expected band size for EIF1 is at 13 kDa.
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ICC/IF analysis of HeLa cells and NIH3T3 cells using GTX54748 eIF1 antibody [2B9]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature
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