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EIF1 Antibody

LS-C497493
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetEIF1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    EIF1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 12kDa, while the observed MW by Western blot was 13kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 12kDa, while the observed MW by Western blot was 13kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10209
  • Target name
    EIF1
  • Target description
    eukaryotic translation initiation factor 1
  • Target synonyms
    A121, EIF-1, EIF1A, ISO1, SUI1, eukaryotic translation initiation factor 1, protein translation factor SUI1 homolog, sui1iso1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EIF1 using anti-EIF1 antibody (A04125-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 7: rat pancreas tissue lysates, Lane 8: mouse pancreas tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF1 antigen affinity purified polyclonal antibody (Catalog # A04125-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF1 at approximately 13 kDa. The expected band size for EIF1 is at 13 kDa.
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