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Western Blot analysis of HeLa cells using PKR Polyclonal Antibody
Western Blot analysis of HeLa cells using PKR Polyclonal Antibody
Western Blot analysis of HeLa cells using PKR Polyclonal Antibody

EIF2AK2 Antibody

CSB-PA003809
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEIF2AK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    EIF2AK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5610
  • Target name
    EIF2AK2
  • Target description
    eukaryotic translation initiation factor 2 alpha kinase 2
  • Target synonyms
    double stranded RNA activated protein kinase; eIF-2A protein kinase 2; EIF2AK1; interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; LEUDEN; P1/eIF-2A protein kinase; p68 kinase; PKR; PPP1R83; PRKR; protein kinase R; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; tyrosine-protein kinase EIF2AK2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP19525
  • Protein Name
    Interferon-induced, double-stranded RNA-activated protein kinase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PKR/EIF2AK2 using anti-PKR/EIF2AK2 antibody (PB9803). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PANC-1 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKR/EIF2AK2 antigen affinity purified polyclonal antibody (Catalog # PB9803) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKR/EIF2AK2 at approximately 69 kDa. The expected band size for PKR/EIF2AK2 is at 62 kDa.
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