
Sample (30 ug of whole cell lysate) A: Raji 5% SDS PAGE GTX118895 diluted at 1:500
EIF3A antibody [N1N2], N-term
GTX118895
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEIF3A
Overview
- SupplierGeneTex
- Product NameEIF3A antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.96 mg/ml
- ConjugateUnconjugated
- Gene ID8661
- Target nameEIF3A
- Target descriptioneukaryotic translation initiation factor 3 subunit A
- Target synonymsEIF3, EIF3S10, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185, eukaryotic translation initiation factor 3 subunit A, EIF3, p180 subunit, centrosomin homolog, cytoplasmic protein p167, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185, eukaryotic translation initiation factor 3 subunit 10, eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD), eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD), eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa, eukaryotic translation initiation factor 3, subunit 10, 170kD
- HostRabbit
- IsotypeIgG
- Protein IDQ14152
- Protein NameEukaryotic translation initiation factor 3 subunit A
- Scientific DescriptionComponent of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![EIF3A antibody [N1N2], N-term detects EIF3A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3A protein stained by EIF3A antibody [N1N2], N-term (GTX118895) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining. EIF3A antibody [N1N2], N-term detects EIF3A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: EIF3A protein stained by EIF3A antibody [N1N2], N-term (GTX118895) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX118895/GTX118895_40310_20150410_IFA_w_23060519_895.webp)








