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EIF3A Antibody

CAB0573
Assay Genie
TargetEIF3A
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    EIF3A Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID8661
  • Target name
    EIF3A
  • Target description
    eukaryotic translation initiation factor 3 subunit A
  • Target synonyms
    EIF3, EIF3S10, P167, TIF32, eIF3-p170, eIF3-theta, p180, p185, eukaryotic translation initiation factor 3 subunit A, EIF3, p180 subunit, centrosomin homolog, cytoplasmic protein p167, eIF-3-theta, eIF3 p167, eIF3 p180, eIF3 p185, eukaryotic translation initiation factor 3 subunit 10, eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD), eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD), eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa, eukaryotic translation initiation factor 3, subunit 10, 170kD
  • Host
    Rabbit
  • Protein IDQ14152
  • Protein Name
    Eukaryotic translation initiation factor 3 subunit A
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Mouse Testis lysates; Lane 2: Human Hela cell lysates probed with eIF3A Polyclonal Antibody, Unconjugated (bs-3840R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of EIF3A using anti-EIF3A antibody (A02339-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: rat L6 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF3A antigen affinity purified polyclonal antibody (Catalog # A02339-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF3A at approximately 180 kDa. The expected band size for EIF3A is at 167 kDa.
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Western Blot Positive WB detected in: Jurkat whole cell lysate All lanes: EIF3A antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 167, 163 kDa Observed band size: 167 kDa
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Immunohistochemical staining of human cerebral cortex, colon, kidney and testis using Anti-EIF3A antibody HPA038315 (A) shows similar protein distribution across tissues to independent antibody HPA038316 (B).
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