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ProductsBack/eIF4E (phospho Ser209) antibody
WB (peptide competition) analysis of HeLa cells using GTX24774 eIF4E (phospho Ser209) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of HeLa cells using GTX24774 eIF4E (phospho Ser209) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.
WB (peptide competition) analysis of HeLa cells using GTX24774 eIF4E (phospho Ser209) antibody prior incubated with the non-phosphopeptide corresponding to the phosphopeptide immunogen (Lane 2), a generic phosphoserine-containing peptide (Lane 3), or the phosphopeptide immunogen (Lane 4) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody. The membrane treated with phosphatase (Lane 5) eliminates the signal further verifying that the antibody is phospho-specific.

eIF4E (phospho Ser209) antibody

GTX24774
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
TargetEIF4E
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Overview

  • Supplier
    GeneTex
  • Product Name
    eIF4E (phospho Ser209) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:250. FACS: 1:20. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1977
  • Target name
    EIF4E
  • Target description
    eukaryotic translation initiation factor 4E
  • Target synonyms
    AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06730
  • Protein Name
    Eukaryotic translation initiation factor 4E
  • Scientific Description
    The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5 end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  • Reactivity
    Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203