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ETFA Antibody (YA3789, Mouse)

HY-P84092
MedChem Express
TargetETFA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ETFA Antibody (YA3789, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2108
  • Target name
    ETFA
  • Target description
    electron transfer flavoprotein subunit alpha
  • Target synonyms
    alpha-ETF; electron transfer flavoprotein alpha subunit; electron transfer flavoprotein subunit alpha, mitochondrial; electron transfer flavoprotein, alpha polypeptide; EMA; epididymis secretory sperm binding protein; GA2; glutaric aciduria II; MADD
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    ETFA Antibody (YA3789) is a mouse-derived and non-conjugated IgG2a antibody, targeting to ETFA. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ETFA using anti-ETFA antibody (A05572-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human U937 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ETFA antigen affinity purified polyclonal antibody (Catalog # A05572-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ETFA at approximately 35 kDa. The expected band size for ETFA is at 35 kDa.
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