More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI5A5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF2S1 (Cat# RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1(Cat# TA501374). Positive lysates [LY401321] (100ug) and [LC401321] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF2S1 (Cat# RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1(Cat# TA501374). Positive lysates [LY401321] (100ug) and [LC401321] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY EIF2S1 (Cat# RC200368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF2S1(Cat# TA501374). Positive lysates [LY401321] (100ug) and [LC401321] (20ug) can be purchased separately from OriGene.

elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI5A5]

CF501374
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetEIF2S1
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    elF2 alpha (EIF2S1) Mouse Monoclonal Antibody [Clone ID: OTI5A5]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5A5
  • Gene ID1965
  • Target name
    EIF2S1
  • Target description
    eukaryotic translation initiation factor 2 subunit alpha
  • Target synonyms
    EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, eukaryotic translation initiation factor 2 subunit 1, eIF-2-alpha, eIF2-alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP05198
  • Protein Name
    Eukaryotic translation initiation factor 2 subunit 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) EIF2S1 mouse monoclonal antibody, clone OTI5A5 (formerly 5A5)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
eIF2alpha AntibodyCAB0764
ReactivityHuman
TargetEIF2S1
  • SizePrice
Western Blot analysis of 3T3 cells using eIF2alpha Polyclonal Antibody
Product group Antibodies
Cusabio
EIF2S1 AntibodyCSB-PA002295
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Monkey, Mouse, Rat
TargetEIF2S1
  • SizePrice
Anti-eIF2 alpha Antibody
Product group Antibodies
Antibodies.com
Anti-eIF2 alpha AntibodyA95082
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-EIF2S1 (Ser51) Antibody (YA203, Rabbit)HY-P80811
TargetEIF2S1
  • SizePrice
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Product group Antibodies
Atlas Antibodies
Anti-EIF2S1 AntibodyHPA064885
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetEIF2S1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
EIF2S1 AntibodyLS-C331101
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetEIF2S1
  • SizePrice
Product group Antibodies
Biomatik
Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1) Polyclonal AntibodyCAU31778
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Rat
TargetEIF2S1
  • SizePrice
Figure 1. Western blot analysis of EIF2S1 using anti-EIF2S1 antibody (P04387). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EIF2S1 antigen affinity purified monoclonal antibody (Catalog # P04387) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EIF2S1 at approximately 36 kDa. The expected band size for EIF2S1 is at 36 kDa.
Product group Antibodies
References
Boster Bio
Anti-Phospho-EIF2S1 (S51) Rabbit Monoclonal AntibodyP04387
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetEIF2S1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-eIF2 alpha Polyclonal Antibody, Unconjugated (bs-3613R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Product group Antibodies
References
Bioss
EIF2S1 Polyclonal AntibodyBS-3613R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Chicken, Human, Mouse, Rabbit, Rat
TargetEIF2S1
  • SizePrice
eIF2 alpha antibody [N1C3] detects eIF2 alpha protein at cytoplasm by immunofluorescent analysis. Sample: HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: eIF2 alpha protein stained by eIF2 alpha antibody [N1C3] (GTX101241) diluted at 1:500. Blue: Hoechst 33342 staining.
Product group Antibodies
GeneTex
eIF2 alpha antibody [N1C3]GTX101241
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetEIF2S1
  • SizePrice