Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Endomucin antibody [N1C2] (GTX101871) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
![Endomucin antibody [N1C2] detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Endomucin antibody [N1C2] (GTX101871) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101871/GTX101871_39609_IHC_w_23060100_105.webp "Endomucin antibody [N1C2] detects Endomucin protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Endomucin antibody [N1C2] (GTX101871) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Endomucin antibody [N1C2] (GTX101871) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
Endomucin antibody [N1C2]
GTX101871
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetEMCN
Overview
- SupplierGeneTex
- Product NameEndomucin antibody [N1C2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID51705
- Target nameEMCN
- Target descriptionendomucin
- Target synonymsEMCN2, MUC14, endomucin, MUC-14, endomucin-2, gastric cancer antigen Ga34, mucin-14
- HostRabbit
- IsotypeIgG
- Protein IDQ9ULC0
- Protein NameEndomucin
- Scientific DescriptionEMCN is a mucin-like sialoglycoprotein that interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix (Kinoshita et al., 2001 [PubMed 11418125]).[supplied by OMIM]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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