Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
Endomucin Polyclonal Antibody
BS-5884R
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat
TargetEMCN
Overview
- SupplierBioss
- Product NameEndomucin Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID51705
- Target nameEMCN
- Target descriptionendomucin
- Target synonymsEMCN2, MUC14, endomucin, MUC-14, endomucin-2, gastric cancer antigen Ga34, mucin-14
- HostRabbit
- IsotypeIgG
- Protein IDQ9ULC0
- Protein NameEndomucin
- ReactivityBovine, Canine, Human, Mouse, Rat
- Storage Instruction-20°C
- UNSPSC41116161
Datasheet
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![Whole cell extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Endomucin antibody [N1C2] (GTX101871) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101871/GTX101871_39609_20201211_WB_w_23060100_940.webp)
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