Paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Endomucin Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining