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Western Blot analysis of HepG2 cells using Enolase Polyclonal Antibody
Western Blot analysis of HepG2 cells using Enolase Polyclonal Antibody
Western Blot analysis of HepG2 cells using Enolase Polyclonal Antibody

ENO2 Antibody

CSB-PA002348
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetENO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ENO2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2026
  • Target name
    ENO2
  • Target description
    enolase 2
  • Target synonyms
    HEL-S-279, NSE, gamma-enolase, 2-phospho-D-glycerate hydro-lyase, 2-phospho-D-glycerate hydrolyase, enolase 2 (gamma, neuronal), epididymis secretory protein Li 279, neural enolase, neuron specific gamma enolase, neuron-specific enolase, neuronal enriched enolase, neurone-specific enolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09104
  • Protein Name
    Gamma-enolase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NSE using anti-NSE antibody (A02930). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysate, Lane 2: human U20S whole cell lysate, Lane 3: human A431 whole cell lysate, Lane 4: human HepG2 whole cell lysate, Lane 5: human A549 whole cell lysate, Lane 6: human SHG-44 whole cell lysate, Lane 7: rat brain tissue lysates, Lane 8: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSE antigen affinity purified polyclonal antibody (Catalog # A02930) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSE at approximately 47KD. The expected band size for NSE is at 47KD.
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