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EP300 Antibody (OAAF01891)

OAAF01891
Aviva Systems Biology
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEP300
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    EP300 Antibody (OAAF01891)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid.PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Gene ID2033
  • Target name
    EP300
  • Target description
    EP300 lysine acetyltransferase
  • Target synonyms
    KAT3B, MKHK2, RSTS2, p300, histone acetyltransferase p300, E1A binding protein p300, E1A-associated protein p300, E1A-binding protein, 300kD, histone butyryltransferase p300, histone crotonyltransferase p300, p300 HAT, protein 2-hydroxyisobutyryltransferase p300, protein lactyltransferas p300, protein propionyltransferase p300
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of KAT3B/P300/EP300 using anti-KAT3B/P300/EP300 antibody (A00117). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT3B/P300/EP300 antigen affinity purified polyclonal antibody (Catalog # A00117) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT3B/P300/EP300 at approximately 264-300 kDa. The expected band size for KAT3B/P300/EP300 is at 264 kDa.
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