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ProductsBack/Eph receptor A5 (EPHA5) Mouse Monoclonal Antibody [Clone ID: 8B10B1]
Western blot analysis using EPHA5 mouse mAb against truncated EPHA5-His recombinant protein (1) and truncated EPHA5 (aa620-774)-hIgGFc transfected CHO-K1 cell lysate (2).
Western blot analysis using EPHA5 mouse mAb against truncated EPHA5-His recombinant protein (1) and truncated EPHA5 (aa620-774)-hIgGFc transfected CHO-K1 cell lysate (2).
Western blot analysis using EPHA5 mouse mAb against truncated EPHA5-His recombinant protein (1) and truncated EPHA5 (aa620-774)-hIgGFc transfected CHO-K1 cell lysate (2).

Eph receptor A5 (EPHA5) Mouse Monoclonal Antibody [Clone ID: 8B10B1]

AM06295SU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetEPHA5
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Eph receptor A5 (EPHA5) Mouse Monoclonal Antibody [Clone ID: 8B10B1]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    8B10B1
  • Gene ID2044
  • Target name
    EPHA5
  • Target description
    EPH receptor A5
  • Target synonyms
    CEK7, EHK-1, EHK1, EK7, HEK7, TYRO4, ephrin type-A receptor 5, EPH homology kinase 1, EPH-like kinase 7, brain-specific kinase, epididymis secretory sperm binding protein, receptor protein-tyrosine kinase HEK7, tyrosine-protein kinase receptor EHK-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP54756
  • Protein Name
    Ephrin type-A receptor 5
  • Scientific Description
    Eph receptor A5 (EPHA5) mouse monoclonal antibody, clone 8B10B1, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph receptor A5 using anti-Eph receptor A5 antibody (PB9583). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug Lane 2: Mouse Brain Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A5 antigen affinity purified polyclonal antibody (Catalog # PB9583) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A5 at approximately 114 kDa. The expected band size for Eph receptor A5 is at 114 kDa.
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