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ProductsBack/Eph receptor B2 (EPHB2) Mouse Monoclonal Antibody [Clone ID: 2D12C6]
Western blot analysis using EphB2 antibody Cat.-No AM06225SU-N against truncated EphB2 recombinant protein (Lane 1) and extracellular EphB2 (aa19-476)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).
Western blot analysis using EphB2 antibody Cat.-No AM06225SU-N against truncated EphB2 recombinant protein (Lane 1) and extracellular EphB2 (aa19-476)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).
Western blot analysis using EphB2 antibody Cat.-No AM06225SU-N against truncated EphB2 recombinant protein (Lane 1) and extracellular EphB2 (aa19-476)-hIgGFc transfected CHO-K1 cell lysate (Lane 2).

Eph receptor B2 (EPHB2) Mouse Monoclonal Antibody [Clone ID: 2D12C6]

AM06225SU-N
OriGene
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetEPHB2
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Eph receptor B2 (EPHB2) Mouse Monoclonal Antibody [Clone ID: 2D12C6]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2D12C6
  • Gene ID2048
  • Target name
    EPHB2
  • Target description
    EPH receptor B2
  • Target synonyms
    BDPLT22, CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5, ephrin type-B receptor 2, EPH-like kinase 5, developmentally-regulated Eph-related tyrosine kinase, elk-related tyrosine kinase, eph tyrosine kinase 3, protein-tyrosine kinase HEK5, renal carcinoma antigen NY-REN-47, tyrosine-protein kinase TYRO5, tyrosine-protein kinase receptor EPH-3
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP29323
  • Protein Name
    Ephrin type-B receptor 2
  • Scientific Description
    Eph receptor B2 (EPHB2) mouse monoclonal antibody, clone 2D12C6, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of EPHB2 using anti-EPHB2 antibody (A01507-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB2 antigen affinity purified polyclonal antibody (Catalog # A01507-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHB2 at approximately 117KD. The expected band size for EPHB2 is at 117KD.
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