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ProductsBack/EPHA1 Antibody (Asp222) (OASG02477)

EPHA1 Antibody (Asp222) (OASG02477)

OASG02477
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetEPHA1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    EPHA1 Antibody (Asp222) (OASG02477)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID2041
  • Target name
    EPHA1
  • Target description
    EPH receptor A1
  • Target synonyms
    EPH, EPHT, EPHT1, ephrin type-A receptor 1, eph tyrosine kinase 1, erythropoietin-producing hepatoma amplified sequence, erythropoietin-producing hepatoma receptor, hEpha1, oncogene EPH, tyrosine-protein kinase receptor EPH
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph receptor A1 using anti-Eph receptor A1 antibody (PA1573). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Colo320 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse lung tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A1 antigen affinity purified polyclonal antibody (Catalog # PA1573) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A1 at approximately 113 kDa. The expected band size for Eph receptor A1 is at 108 kDa.
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