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Western Blot analysis of 3T3 cells using EphA2/3/4 Polyclonal Antibody
Western Blot analysis of 3T3 cells using EphA2/3/4 Polyclonal Antibody
Western Blot analysis of 3T3 cells using EphA2/3/4 Polyclonal Antibody

EPHA2/EPHA3/EPHA4 Antibody

CSB-PA002362
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetEPHA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    EPHA2/EPHA3/EPHA4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1969
  • Target name
    EPHA2
  • Target description
    EPH receptor A2
  • Target synonyms
    ARCC2, CTPA, CTPP1, CTRCT6, ECK, ephrin type-A receptor 2, epithelial cell receptor protein tyrosine kinase, tyrosine-protein kinase receptor ECK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29317
  • Protein Name
    Ephrin type-A receptor 2
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Eph receptor A2 using anti-Eph receptor A2 antibody (A00578). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela cell lysate, Lane 2: human U-87MG cell lysate, Lane 3: human SHG-44 cell lysate, Lane 4: human COLO-320 cell lysate, Lane 5: human SK-OV-3 cell lysate, Lane 6: human A549 cell lysate, Lane 7: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor A2 antigen affinity purified polyclonal antibody (Catalog # A00578) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor A2 at approximately 125KD. The expected band size for Eph receptor A2 is at 108KD.
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