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WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB1 (Lane 2) using GTX81349 EphB1 antibody.
WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB1 (Lane 2) using GTX81349 EphB1 antibody.
WB analysis of 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EphB1 (Lane 2) using GTX81349 EphB1 antibody.

EphB1 antibody

GTX81349
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetEPHB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    EphB1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2047
  • Target name
    EPHB1
  • Target description
    EPH receptor B1
  • Target synonyms
    EK6; ELK; eph tyrosine kinase 2; EPH-like kinase 6; ephrin type-B receptor 1; EPHT2; Hek6; NET; neuronally-expressed EPH-related tyrosine kinase; soluble EPHB1 variant 1; tyrosine-protein kinase receptor EPH-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54762
  • Protein Name
    Ephrin type-B receptor 1
  • Scientific Description
    Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Eph receptor B1 using anti-Eph receptor B1 antibody (PB9584). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Hel whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Eph receptor B1 antigen affinity purified polyclonal antibody (Catalog # PB9584) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Eph receptor B1 at approximately 120 kDa. The expected band size for Eph receptor B1 is at 110 kDa.
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