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IHC-P analysis of human lung squamous cell carcinoma (A), lung adenocarcinoma (B), colon carcinoma (C), breast carcinoma (D), normal sublingual gland (E), normal rectal (F) using GTX83041 EphB3 antibody [4A122D1].
IHC-P analysis of human lung squamous cell carcinoma (A), lung adenocarcinoma (B), colon carcinoma (C), breast carcinoma (D), normal sublingual gland (E), normal rectal (F) using GTX83041 EphB3 antibody [4A122D1].
IHC-P analysis of human lung squamous cell carcinoma (A), lung adenocarcinoma (B), colon carcinoma (C), breast carcinoma (D), normal sublingual gland (E), normal rectal (F) using GTX83041 EphB3 antibody [4A122D1].

EphB3 antibody [4A122D1]

GTX83041
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEPHB3
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Overview

  • Supplier
    GeneTex
  • Product Name
    EphB3 antibody [4A122D1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4A122D1
  • Conjugate
    Unconjugated
  • Gene ID2049
  • Target name
    EPHB3
  • Target description
    EPH receptor B3
  • Target synonyms
    EK2; embryonic kinase 2; EPH-like tyrosine kinase 2; ephrin type-B receptor 3; ETK2; HEK2; human embryo kinase 2; TYRO6; tyrosine-protein kinase TYRO6
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP54753
  • Protein Name
    Ephrin type-B receptor 3
  • Scientific Description
    Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EPHB3 using anti-EPHB3 antibody (A04659). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat lung tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPHB3 antigen affinity purified polyclonal antibody (Catalog # A04659) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPHB3 at approximately 110 kDa. The expected band size for EPHB3 is at 110-130 kDa.
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