More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ERM antibody
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ERM antibody (GTX114394) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERM antibody

GTX114394
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetETV5
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    ERM antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.48 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2119
  • Target name
    ETV5
  • Target description
    ETS variant transcription factor 5
  • Target synonyms
    ERM, ETS translocation variant 5, ets-related molecule, ets-related protein ERM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41161
  • Protein Name
    ETS translocation variant 5
  • Scientific Description
    Binds to DNA sequences containing the consensus nucleotide core sequence GGAA.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: ETV5 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 63 kDa Observed band size: 58 kDa
Product group Antibodies
Cusabio
ETV5 AntibodyCSB-PA007859LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetETV5
  • SizePrice
Figure 1. Western blot analysis of ERM/Etv5 using anti-ERM/Etv5 antibody (A01809-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERM/Etv5 antigen affinity purified polyclonal antibody (Catalog # A01809-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERM/Etv5 at approximately 70KD. The expected band size for ERM/Etv5 is at 70KD.
Product group Antibodies
Boster Bio
Anti-ERM/Etv5 Antibody Picoband(r)A01809-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetETV5
  • SizePrice
Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
Anti-ETV5 AntibodyHPA063065
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetETV5
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ETV5 / ERM Antibody (FITC)LS-C680427
ApplicationsELISA
ReactivityHuman
TargetETV5
  • SizePrice
Product group Antibodies
Biomatik
ETV5 Polyclonal AntibodyCAC15424
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetETV5
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Etv5 Polyclonal Antibody (bs-8592R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
Bioss
Etv5 Polyclonal AntibodyBS-8592R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetETV5
  • SizePrice
WB analysis of Jurkat (1), NIH3T3 (2) and MCF-7 (3) cell lysate using GTX82825 ERM antibody [3H3].
Product group Antibodies
GeneTex
ERM antibody [3H3]GTX82825
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse
TargetETV5
  • SizePrice
Product group Antibodies
Biosynth
ETV5 antibody10R-2196
  • SizePrice
Product group Antibodies
MedChem Express
ERM/Etv5 Antibody (YA3998, Mouse)HY-P84301
TargetETV5
  • SizePrice