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ERM/Etv5 Antibody (YA3998, Mouse)

HY-P84301
MedChem Express
TargetETV5
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    ERM/Etv5 Antibody (YA3998, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2119
  • Target name
    ETV5
  • Target description
    ETS variant transcription factor 5
  • Target synonyms
    ERM, ETS translocation variant 5, ets-related molecule, ets-related protein ERM
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    ERM/Etv5 Antibody (YA3998) is a mouse-derived and non-conjugated IgG1 antibody, targeting to ETV5. It can be applicated for WB, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: ETV5 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 63 kDa Observed band size: 58 kDa
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Figure 1. Western blot analysis of ERM/Etv5 using anti-ERM/Etv5 antibody (A01809-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERM/Etv5 antigen affinity purified polyclonal antibody (Catalog # A01809-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERM/Etv5 at approximately 70KD. The expected band size for ERM/Etv5 is at 70KD.
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