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ETV5 Polyclonal Antibody

CAC15424
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetETV5
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Overview

  • Supplier
    Biomatik
  • Product Name
    ETV5 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2119
  • Target name
    ETV5
  • Target description
    ETS variant transcription factor 5
  • Target synonyms
    ERM, ETS translocation variant 5, ets-related molecule, ets-related protein ERM
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP41161
  • Protein Name
    ETS translocation variant 5
  • Scientific Description
    The ETV5 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: PC-3 whole cell lysate, A549 whole cell lysate All lanes: ETV5 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 63 kDa Observed band size: 58 kDa
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Figure 1. Western blot analysis of ERM/Etv5 using anti-ERM/Etv5 antibody (A01809-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Neuro-2a whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERM/Etv5 antigen affinity purified polyclonal antibody (Catalog # A01809-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERM/Etv5 at approximately 70KD. The expected band size for ERM/Etv5 is at 70KD.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Etv5 Polyclonal Antibody (bs-8592R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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