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ERP29 Antibody Pair

CSB-EAP05345
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Human, Porcine, Sheep
TargetERP29
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Overview

  • Supplier
    Cusabio
  • Product Name
    ERP29 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 1ug/ml and the detection antibody at a concentration of 0.5ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID10961
  • Target name
    ERP29
  • Target description
    endoplasmic reticulum protein 29
  • Target synonyms
    C12orf8, ERp28, ERp31, HEL-S-107, PDI-DB, PDIA9, endoplasmic reticulum resident protein 29, endoplasmic reticulum lumenal protein ERp28, endoplasmic reticulum resident protein 28, endoplasmic reticulum resident protein 31, epididymis secretory protein Li 107, protein disulfide isomerase family A, member 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30040
  • Protein Name
    Endoplasmic reticulum resident protein 29
  • Reactivity
    Bovine, Canine, Guinea Pig, Human, Porcine, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of ERP29 using anti-ERP29 antibody (A03621-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human placenta tissue lysates, Lane 7: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERP29 antigen affinity purified polyclonal antibody (Catalog # A03621-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERP29 at approximately 29 kDa. The expected band size for ERP29 is at 29 kDa.
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