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Estrogen Receptor beta Antibody (YA1892, Rabbit)

HY-P82147
MedChem Express
TargetESR2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Estrogen Receptor beta Antibody (YA1892, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2100
  • Target name
    ESR2
  • Target description
    estrogen receptor 2
  • Target synonyms
    ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8, estrogen receptor beta, estrogen receptor beta 2, estrogen receptor beta 4, nuclear receptor subfamily 3 group A member 2, oestrogen receptor beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Estrogen Receptor beta Antibody (YA1892) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1892), targeting Estrogen Receptor beta, with a predicted molecular weight of 59 kDa (observed band size: 59 kDa). Estrogen Receptor beta Antibody (YA1892) can be used for WB, IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
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