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EZH1 Rabbit pAb, FITC conjugated

ORB102559
Biorbyt
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, Canine, Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
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Overview

  • Supplier
    Biorbyt
  • Product Name
    EZH1 Rabbit pAb, FITC conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    Flow-Cyt=2ug/Test, IF=1:100-500, ICC/IF=1:50-200 FC, ICC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    FITC
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    EZH1 Rabbit pAb, FITC conjugated
  • Reactivity
    Bovine, Canine, Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of EZH1 using anti-EZH1 antibody (A02494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH1 antigen affinity purified polyclonal antibody (Catalog # A02494-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EZH1 at approximately 100 kDa, 60 kDa. The expected band size for EZH1 is at 68 kDa.
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