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Western Blot analysis of HeLa cells using Thrombin R Polyclonal Antibody
Western Blot analysis of HeLa cells using Thrombin R Polyclonal Antibody
Western Blot analysis of HeLa cells using Thrombin R Polyclonal Antibody

F2R Antibody

CSB-PA004289
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetF2R
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Overview

  • Supplier
    Cusabio
  • Product Name
    F2R Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2149
  • Target name
    F2R
  • Target description
    coagulation factor II thrombin receptor
  • Target synonyms
    CF2R; HTR; PAR1; PAR-1; protease-activated receptor 1; proteinase-activated receptor 1; TR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25116
  • Protein Name
    Proteinase-activated receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of F2R using anti-F2R antibody (A03352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2R antigen affinity purified polyclonal antibody (Catalog # A03352-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for F2R at approximately 47KD. The expected band size for F2R is at 47KD.
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