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ProductsBack/Goat anti-F2R / PAR1

Goat anti-F2R / PAR1

EB09268
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetF2R
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-F2R / PAR1 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2149
  • Target name
    F2R
  • Target description
    coagulation factor II thrombin receptor
  • Target synonyms
    CF2R; HTR; PAR1; PAR-1; protease-activated receptor 1; proteinase-activated receptor 1; TR
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001983.2. Purification: Antigen affinity purified. Names and symbols: F2R; coagulation factor II (thrombin) receptor; CF2R; HTR; PAR1; TR; coagulation factor II receptor; protease-activated receptor 1; thrombin receptor
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of F2R using anti-F2R antibody (A03352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2R antigen affinity purified polyclonal antibody (Catalog # A03352-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for F2R at approximately 47KD. The expected band size for F2R is at 47KD.
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