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ProductsBack/PAR1 Polyclonal Antibody
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-PAR-1 Polyclonal Antibody (bs-0828R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-PAR-1 Polyclonal Antibody (bs-0828R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-PAR-1 Polyclonal Antibody (bs-0828R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

PAR1 Polyclonal Antibody

BS-0828R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetF2R
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Thrombin receptor Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2149
  • Target name
    F2R
  • Target description
    coagulation factor II thrombin receptor
  • Target synonyms
    CF2R; HTR; PAR1; PAR-1; protease-activated receptor 1; proteinase-activated receptor 1; TR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25116
  • Protein Name
    Proteinase-activated receptor 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • The Synthetic Curcumin Derivative CNB-001 Attenuates Thrombin-Stimulated Microglial Inflammation by Inhibiting the ERK and p38 MAPK Pathways. Akaishi T et al., 2020, Biol Pharm Bull
    Read more
  • Antiapoptotic Effect by PAR-1 Antagonist Protects Mouse Liver Against Ischemia-Reperfusion Injury. Noguchi D et al., 2020 Feb, J Surg Res
    Read more
  • Protease-activated receptors are potential regulators in the development of arterial endofibrosis in high-performance athletes. Posthuma JJ et al., 2019 Apr, J Vasc Surg
    Read more
  • Recovery from trauma induced amnesia correlates with normalization of thrombin activity in the mouse hippocampus. Ben Shimon M et al., 2017, PLoS One
    Read more

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Figure 1. Western blot analysis of F2R using anti-F2R antibody (A03352-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-F2R antigen affinity purified polyclonal antibody (Catalog # A03352-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for F2R at approximately 47KD. The expected band size for F2R is at 47KD.
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