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ProductsBack/FAAH1 Rabbit mAb

FAAH1 Rabbit mAb

A4099
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFAAH
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Overview

  • Supplier
    ABclonal
  • Product Name
    FAAH1 Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2166
  • Target name
    FAAH
  • Target description
    fatty acid amide hydrolase
  • Target synonyms
    FAAH-1, FAAH1, PSAB, fatty-acid amide hydrolase 1, anandamide amidohydrolase 1, fatty acid ester hydrolase, oleamide hydrolase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00519
  • Protein Name
    Fatty-acid amide hydrolase 1
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier Note
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FAAH1/FAAH using anti-FAAH1/FAAH antibody (A00801-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAAH1/FAAH antigen affinity purified polyclonal antibody (Catalog # A00801-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAAH1/FAAH at approximately 63 kDa. The expected band size for FAAH1/FAAH is at 63 kDa.
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