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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA007955ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA007955ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA007955ESR1HU at dilution of 1:100

FABP6 Antibody

CSB-PA007955ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFABP6
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Overview

  • Supplier
    Cusabio
  • Product Name
    FABP6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2172
  • Target name
    FABP6
  • Target description
    fatty acid binding protein 6
  • Target synonyms
    fatty acid binding protein 6, ileal; gastrotropin; GT; I-15P; I-BABP; I-BALB; I-BAP; ILBP; ILBP3; ileal bile acid binding protein; ileal lipid-binding protein; ILLBP; illeal lipid-binding protein; intestinal 15 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51161
  • Protein Name
    Gastrotropin
  • Scientific Description
    Ileal protein which stimulates gastric acid and pepsinogen secretion. Seems to be able to bind to bile salts and bilirubins. Isoform 2 is essential for the survival of colon cancer cells to bile acid-induced apoptosis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FABP6 using anti-FABP6 antibody (A07132). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat intestine tissue lysates, Lane 2: rat intestine tissue lysates, Lane 3: mouse intestine tissue lysates, Lane 4: mouse intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP6 antigen affinity purified polyclonal antibody (Catalog # A07132) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP6 at approximately 14KD. The expected band size for FABP6 is at 14KD.
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