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ProductsBack/I-BABP / FABP6 Antibody

I-BABP / FABP6 Antibody

LS-C401653
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFABP6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    I-BABP / FABP6 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, IHC (1:50 - 1:200), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2172
  • Target name
    FABP6
  • Target description
    fatty acid binding protein 6
  • Target synonyms
    I-15P, I-BABP, I-BALB, I-BAP, ILBP, ILBP3, ILLBP, gastrotropin, GT, fatty acid binding protein 6, ileal, ileal bile acid binding protein, ileal lipid-binding protein, illeal lipid-binding protein, intestinal 15 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FABP6 using anti-FABP6 antibody (A07132). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat intestine tissue lysates, Lane 2: rat intestine tissue lysates, Lane 3: mouse intestine tissue lysates, Lane 4: mouse intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP6 antigen affinity purified polyclonal antibody (Catalog # A07132) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP6 at approximately 14KD. The expected band size for FABP6 is at 14KD.
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