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ILBP antibody

GTX105556
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFABP6
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Overview

  • Supplier
    GeneTex
  • Product Name
    ILBP antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2172
  • Target name
    FABP6
  • Target description
    fatty acid binding protein 6
  • Target synonyms
    fatty acid binding protein 6, ileal; gastrotropin; GT; I-15P; I-BABP; I-BALB; I-BAP; ILBP; ILBP3; ileal bile acid binding protein; ileal lipid-binding protein; ILLBP; illeal lipid-binding protein; intestinal 15 kDa protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51161
  • Protein Name
    Gastrotropin
  • Scientific Description
    This gene encodes the ileal fatty acid binding protein. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABP6 and FABP1 (the liver fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Transcript variants generated by alternate transcription promoters and/or alternate splicing have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FABP6 using anti-FABP6 antibody (A07132). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat intestine tissue lysates, Lane 2: rat intestine tissue lysates, Lane 3: mouse intestine tissue lysates, Lane 4: mouse intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FABP6 antigen affinity purified polyclonal antibody (Catalog # A07132) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FABP6 at approximately 14KD. The expected band size for FABP6 is at 14KD.
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