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Western Blot analysis of Hela cell using FAF1 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Hela cell using FAF1 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Hela cell using FAF1 Polyclonal Antibody at dilution of 1:400

FAF1 Polyclonal Antibody

E-AB-15629
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    FAF1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.9mg/ml
  • Scientific Description
    Interaction of Fas ligand (TNFSF6) with the FAS antigen (TNFRSF6) mediates programmed cell death, also called apoptosis, in a number of organ systems. The protein encoded by this gene binds to FAS antigen and can initiate apoptosis or enhance apoptosis initiated through FAS antigen. Initiation of apoptosis by the protein encoded by this gene requires a ubiquitin-like domain but not the FAS-binding domain.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FAF1 using anti-FAF1 antibody (A03842-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAF1 antigen affinity purified polyclonal antibody (Catalog # A03842-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAF1 at approximately 74 kDa. The expected band size for FAF1 is at 74 kDa.
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