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ProductsBack/FAN antibody [N2C1], Internal

FAN antibody [N2C1], Internal

GTX103723
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNSMAF
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Overview

  • Supplier
    GeneTex
  • Product Name
    FAN antibody [N2C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.93 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8439
  • Target name
    NSMAF
  • Target description
    neutral sphingomyelinase activation associated factor
  • Target synonyms
    factor associated with neutral sphingomyelinase activation; factor associated with N-SMase activation; FAN; GRAMD5; neutral sphingomyelinase (N-SMase) activation associated factor; protein FAN
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92636
  • Protein Name
    Protein FAN
  • Scientific Description
    This gene encodes a WD-repeat protein that binds the cytoplasmic sphingomyelinase activation domain of the 55kD tumor necrosis factor receptor. This protein is required for TNF-mediated activation of neutral sphingomyelinase and may play a role in regulating TNF-induced cellular responses such as inflammation. Alternative splicing results in multiple transcript variants.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSMAF using anti-NSMAF antibody (A09560). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSMAF antigen affinity purified polyclonal antibody (A09560) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSMAF at approximately 104 kDa. The expected band size for NSMAF is at 104 kDa.
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