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FAT10/UBD Rabbit pAb

A5491
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetUBD
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Overview

  • Supplier
    ABclonal
  • Product Name
    FAT10/UBD Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    WB,IF/ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10537
  • Target name
    UBD
  • Target description
    ubiquitin like modifier D
  • Target synonyms
    FAT10, UBD-3, ubiquitin D, HLA-F adjacent transcript 10, diubiquitin, ubiquitin-like protein FAT10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15205
  • Protein Name
    Ubiquitin D
  • Reactivity
    Human, Mouse
  • Reactivity Supplier Note
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UBD using anti-UBD antibody (A01970-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBD antigen affinity purified polyclonal antibody (Catalog # A01970-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBD at approximately 18KD. The expected band size for UBD is at 18KD.
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