Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase(GTX110470) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
![Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)](https://www.genetex.com/upload/website/prouct_img/normal/GTX110470/GTX110470_40037_20191115_WB_treatment_M_Stimulated_w_23060500_188.webp "Unstimulatd and stimulatd 3T3-L1 whole cell extracts (20 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The differentiation stimulated medium is composed by basal medium, 10% FBS, 50 ug/ml gentamicin, 1 nM L-glutamin, 500 uM IBMX, 1 uM dexamethasone, 2 uM rosiglitazone and 1 ug/ml insulin.)")
![Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110470/GTX110470_40037_20191101_IHC-P_M_1_w_23060500_143.webp "Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:3000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110470/GTX110470_40037_20160616_WB_shRNA_watermark_w_23060500_663.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:3000.")
![Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110470/GTX110470_41780_20150601_IFA_w_23060500_939.webp "Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
![Immunoprecipitation of Fatty Acid Synthase protein from HeLa whole cell extracts using 5 μg of Fatty Acid Synthase antibody [N1N2], N-term (GTX110470). Western blot analysis was performed using Fatty Acid Synthase antibody [N1N2], N-term (GTX110470). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110470/GTX110470_40037_20150710_IP_w_23060500_786.webp "Immunoprecipitation of Fatty Acid Synthase protein from HeLa whole cell extracts using 5 μg of Fatty Acid Synthase antibody [N1N2], N-term (GTX110470). Western blot analysis was performed using Fatty Acid Synthase antibody [N1N2], N-term (GTX110470). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX110470/GTX110470_40037_20191101_IHC-P_M_w_23060500_525.webp "Fatty Acid Synthase antibody [N1N2], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse adrenal gland. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1N2], N-term (GTX110470) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Fatty Acid Synthase(GTX110470) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Fatty Acid Synthase antibody [N1N2], N-term
GTX110470
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetFASN
Overview
- SupplierGeneTex
- Product NameFatty Acid Synthase antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2194
- Target nameFASN
- Target descriptionfatty acid synthase
- Target synonymsFAS, OA-519, SDR27X1, fatty acid synthase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-[acyl-carrier-protein] synthase, Acyl-[acyl-carrier-protein] hydrolase, Enoyl-[acyl-carrier-protein] reductase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, short chain dehydrogenase/reductase family 27X, member 1, type I fatty acid synthase
- HostRabbit
- IsotypeIgG
- Protein IDP49327
- Protein NameFatty acid synthase
- Scientific DescriptionThe enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- You BJ, Chen LY, Hsu PH, et al. Orlistat Displays Antitumor Activity and Enhances the Efficacy of Paclitaxel in Human Hepatoma Hep3B Cells. Chem Res Toxicol. 2019,32(2):255-264. doi: 10.1021/acs.chemrestox.8b00269Read this paper
Datasheet
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