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Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody
Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody
Western blot analysis of extract from 293, Hela cells using Fibrillarin Antibody

FBL Antibody

CSB-PA919400
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFBL
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Overview

  • Supplier
    Cusabio
  • Product Name
    FBL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2091
  • Target name
    FBL
  • Target description
    fibrillarin
  • Target synonyms
    34 kDa nucleolar scleroderma antigen; 34-kD nucleolar scleroderma antigen; FIB; FLRN; histone-glutamine methyltransferase; Nop1; RNA, U3 small nucleolar interacting protein 1; RNU3IP1; rRNA 2'-O-methyltransferase fibrillarin; U6 snRNA 2'-O-methyltransferase fibrillarin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22087
  • Protein Name
    rRNA 2'-O-methyltransferase fibrillarin
  • Scientific Description
    Involved in pre-rRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Lischwe M.A., Ochs R.L., Reddy R., Cook R.G., Yeoman L.C., Tan E.M., Reichlin M., Busch H. J. Biol. Chem. 260:14304-14310(1985) Yanagida M., Hayano T., Yamauchi Y., Shinkawa T., Natsume T., Isobe T., Takahashi N.J. Biol. Chem. 279:1607-1614(2004) Wang Y., Liu J., Zhao H., Lue W., Zhao J., Yang L., Li N., Du X., Ke Y.Biochim. Biophys. Acta 1773:863-868(2007)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Fibrillarin/FBL using anti-Fibrillarin/FBL antibody (A03178-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrillarin/FBL antigen affinity purified polyclonal antibody (Catalog # A03178-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrillarin/FBL at approximately 37 kDa. The expected band size for Fibrillarin/FBL is at 37 kDa.
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