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ProductsBack/FER antibody [5D2C4]
ICC/IF analysis of HeLa cells using GTX83329 FER antibody [5D2C4]. Green : FER Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of HeLa cells using GTX83329 FER antibody [5D2C4]. Green : FER Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments
ICC/IF analysis of HeLa cells using GTX83329 FER antibody [5D2C4]. Green : FER Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments

FER antibody [5D2C4]

GTX83329
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFER
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Overview

  • Supplier
    GeneTex
  • Product Name
    FER antibody [5D2C4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5D2C4
  • Conjugate
    Unconjugated
  • Gene ID2241
  • Target name
    FER
  • Target description
    FER tyrosine kinase
  • Target synonyms
    PPP1R74, TYK3, p94-Fer, tyrosine-protein kinase Fer, feline encephalitis virus-related kinase FER, fer (fps/fes related) tyrosine kinase, fujinami poultry sarcoma/Feline sarcoma-related protein Fer, phosphoprotein NCP94, protein phosphatase 1, regulatory subunit 74, proto-oncogene c-Fer, tyrosine kinase 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16591
  • Protein Name
    Tyrosine-protein kinase Fer
  • Scientific Description
    The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FER using anti-FER antibody (A01630-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FER antigen affinity purified polyclonal antibody (Catalog # A01630-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FER at approximately 95 kDa. The expected band size for FER is at 95 kDa.
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