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ProductsBack/FER (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6B9]
Detection of endogenous fer: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab fer-6B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment: 15 min. Lane 1: A431; Lane 2: SW480 lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB231; lane 7: T47D
Detection of endogenous fer: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab fer-6B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment: 15 min. Lane 1: A431; Lane 2: SW480 lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB231; lane 7: T47D
Detection of endogenous fer: Whole cell extracts of pervanadate (VH) treated tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab fer-6B9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). VH treatment: 15 min. Lane 1: A431; Lane 2: SW480 lane 3: SW620; lane 4: HT29; lane 5: MCF-7; lane 6: MDA-MB231; lane 7: T47D

FER (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6B9]

AM00060PU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetFER
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FER (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6B9]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6B9
  • Gene ID2241
  • Target name
    FER
  • Target description
    FER tyrosine kinase
  • Target synonyms
    PPP1R74, TYK3, p94-Fer, tyrosine-protein kinase Fer, feline encephalitis virus-related kinase FER, fer (fps/fes related) tyrosine kinase, fujinami poultry sarcoma/Feline sarcoma-related protein Fer, phosphoprotein NCP94, protein phosphatase 1, regulatory subunit 74, proto-oncogene c-Fer, tyrosine kinase 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16591
  • Protein Name
    Tyrosine-protein kinase Fer
  • Scientific Description
    FER (incl. pos. control) mouse monoclonal antibody, clone 6B9, Purified
  • Reactivity
    Canine, Human, Mouse, Rat
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FER using anti-FER antibody (A01630-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FER antigen affinity purified polyclonal antibody (Catalog # A01630-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FER at approximately 95 kDa. The expected band size for FER is at 95 kDa.
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