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Western Blot analysis of Mouse kidney tissue using FGF9 Polyclonal Antibody at dilution of 1:800
Western Blot analysis of Mouse kidney tissue using FGF9 Polyclonal Antibody at dilution of 1:800
Western Blot analysis of Mouse kidney tissue using FGF9 Polyclonal Antibody at dilution of 1:800

FGF9 Polyclonal Antibody

E-AB-10180
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    FGF9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGF9 using anti-FGF9 antibody (A02274). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF9 antigen affinity purified polyclonal antibody (Catalog # A02274) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGF9 at approximately 26KD. The expected band size for FGF9 is at 23KD.
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