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ProductsBack/FGFR1 Antibody - middle region (OAAB18894)

FGFR1 Antibody - middle region (OAAB18894)

OAAB18894
Aviva Systems Biology
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetFGFR1
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    FGFR1 Antibody - middle region (OAAB18894)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Format
    Liquid PBS with 0.09% (W/V) sodium azide.
  • Gene ID2260
  • Target name
    FGFR1
  • Target description
    fibroblast growth factor receptor 1
  • Target synonyms
    BFGFR, CD331, CEK, ECCL, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD, bFGF-R-1, fibroblast growth factor receptor 1, FGFR1/PLAG1 fusion, FMS-like tyrosine kinase 2, basic fibroblast growth factor receptor 1, fms-related tyrosine kinase 2, heparin-binding growth factor receptor, hydroxyaryl-protein kinase, proto-oncogene c-Fgr
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGFR1 using anti-FGFR1 antibody (A00098). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR1 antigen affinity purified polyclonal antibody (Catalog # A00098) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR1 at approximately 100KD. The expected band size for FGFR1 is at 91KD.
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