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ProductsBack/FGFR1 / FGF Receptor 1 Antibody (aa22-376)

FGFR1 / FGF Receptor 1 Antibody (aa22-376)

LS-C812375
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFGFR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FGFR1 / FGF Receptor 1 Antibody (aa22-376)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2260
  • Target name
    FGFR1
  • Target description
    fibroblast growth factor receptor 1
  • Target synonyms
    basic fibroblast growth factor receptor 1; BFGFR; bFGF-R-1; CD331; CEK; ECCL; FGFBR; FGFR-1; FGFR1/PLAG1 fusion; fibroblast growth factor receptor 1; FLG; FLT2; FLT-2; FMS-like tyrosine kinase 2; fms-related tyrosine kinase 2; HBGFR; heparin-binding growth factor receptor; HH2; HRTFDS; hydroxyaryl-protein kinase; KAL2; N-SAM; OGD; proto-oncogene c-Fgr
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FGFR1 using anti-FGFR1 antibody (A00098). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR1 antigen affinity purified polyclonal antibody (Catalog # A00098) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR1 at approximately 100KD. The expected band size for FGFR1 is at 91KD.
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