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FHIT Antibody Pair

CSB-EAP11547
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityAvian, Bovine, Canine, Equine, Guinea Pig, Goat, Human, Porcine
TargetFHIT
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Overview

  • Supplier
    Cusabio
  • Product Name
    FHIT Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.5ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID2272
  • Target name
    FHIT
  • Target description
    fragile histidine triad diadenosine triphosphatase
  • Target synonyms
    AP3A hydrolase; AP3Aase; bis(5'-adenosyl)-triphosphatase; diadenosine 5',5'''-P1,P3-triphosphate hydrolase; dinucleosidetriphosphatase; FRA3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49789
  • Protein Name
    Bis(5'-adenosyl)-triphosphatase
  • Reactivity
    Avian, Bovine, Canine, Equine, Guinea Pig, Goat, Human, Porcine
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of FHIT using anti-FHIT antibody (PB9181). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHIT antigen affinity purified polyclonal antibody (Catalog # PB9181) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHIT at approximately 17 kDa. The expected band size for FHIT is at 17 kDa.
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