![WB analysis of 25 ug of human plasma lysates using GTX15737 Fibronectin antibody [3F12]. Dilution : 1:300 WB analysis of 25 ug of human plasma lysates using GTX15737 Fibronectin antibody [3F12]. Dilution : 1:300](https://www.genetex.com/upload/website/prouct_img/normal/GTX15737/GTX15737_1519_WB_w_23060620_400.webp)
WB analysis of 25 ug of human plasma lysates using GTX15737 Fibronectin antibody [3F12]. Dilution : 1:300
Fibronectin antibody [3F12]
GTX15737
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, RadioImmunoAssay
Product group Antibodies
TargetFN1
Overview
- SupplierGeneTex
- Product NameFibronectin antibody [3F12]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:100-1:500. ELISA: 1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, RadioImmunoAssay
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3F12
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2335
- Target nameFN1
- Target descriptionfibronectin 1
- Target synonymsCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF, fibronectin, cold-insoluble globulin, epididymis secretory sperm binding protein, lnc-ABCA12-8, migration-stimulating factor
- HostMouse
- IsotypeIgG1
- Protein IDP02751
- Protein NameFibronectin
- Scientific DescriptionThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016]
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![WB analysis of 30 microg of the Caco-2 whole cell lysates using GTX15737 Fibronectin antibody [3F12]. Dilution : 2 microg/ml WB analysis of 30 microg of the Caco-2 whole cell lysates using GTX15737 Fibronectin antibody [3F12]. Dilution : 2 microg/ml](https://www.genetex.com/upload/website/prouct_img/normal/GTX15737/GTX15737_1520_WB_w_23060620_590.webp)
![ICC/IF analysis of HepG2 cells using GTX15737 Fibronectin antibody [3F12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 overnight at 4 oC ICC/IF analysis of HepG2 cells using GTX15737 Fibronectin antibody [3F12]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX15737/GTX15737_291_ICC-IF_w_23060620_643.webp)
![Sandwich ELISA analysis of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml, 100ul) using GTX15737 Fibronectin antibody [3F12] (detection antibody) at 1 microg/mL and fibronectin polyclonal antibody (capture antibody). Sandwich ELISA analysis of recombinant human plasma fibronectin protein ranging from 1.6-1000ng/ml, 100ul) using GTX15737 Fibronectin antibody [3F12] (detection antibody) at 1 microg/mL and fibronectin polyclonal antibody (capture antibody).](https://www.genetex.com/upload/website/prouct_img/normal/GTX15737/GTX15737_40_ELISA_w_23060620_853.webp)

![ELISA analysis of antigen using GTX60570 Fibronectin antibody [2F4]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60570/GTX60570_20170912_ELISA_w_23061123_430.webp)
![ELISA analysis of antigen using GTX60571 Fibronectin antibody [2F4G2]. Black : Control antigen 100ng Purple : Antigen 10ng Blue : Antigen 50ng Red : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60571/GTX60571_20170912_ELISA_w_23061123_827.webp)
![IHC-P analysis of human pancreatic adenocarcinoma tissue using GTX34725 Fibronectin antibody [HFN7.1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34725/GTX34725_20200115_IHC-P_908_w_23060801_383.webp)
![IHC-P analysis of human pancreatic adenocarcinoma tissue using GTX34726 Fibronectin antibody [SPM539].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34726/GTX34726_20200115_IHC-P_910_w_23060801_579.webp)