ProductsBack/Filaggrin antibody [FLG01]

The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Filaggrin antibody [FLG01]

GTX23137

ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityHuman, Mouse

TargetFLG

Overview

Supplier
GeneTex
Product Name
Filaggrin antibody [FLG01]
Delivery Days Customer
9
Application Supplier Note
Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4microg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Applications
ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
FLG01
Concentration
0.2 mg/ml
Conjugate
Unconjugated
Gene ID2312
Target name
FLG
Target description
filaggrin
Target synonyms
ATOD2, FLG-1, FLG1, filaggrin, epidermal filaggrin
Host
Mouse
Isotype
IgG1
Protein IDP20930
Protein Name
Filaggrin
Scientific Description
The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]
Reactivity
Human, Mouse
Storage Instruction
2°C to 8°C
UNSPSC
12352203

Oh JS, Lee SJ, Choung SY. Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice. Nutrients. 2021,13(9). doi: 10.3390/nu13093209
Read this paper
Gutowska-Owsiak D, de La Serna JB, Fritzsche M, et al. Orchestrated control of filaggrin-actin scaffolds underpins cornification. Cell Death Dis. 2018,9(4):412. doi: 10.1038/s41419-018-0407-2
Read this paper
Kolesnik M, Franke I, Lux A, et al. Eczema in Psoriatico: An Important Differential Diagnosis Between Chronic Allergic Contact Dermatitis and Psoriasis in Palmoplantar Localization. Acta Derm Venereol. 2018,98(1):50-58. doi: 10.2340/00015555-2779
Read this paper
Naeem AS, Tommasi C, Cole C, et al. A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic dermatitis. J Allergy Clin Immunol. 2017,139(4):1228-1241. doi: 10.1016/j.jaci.2016.09.052
Read this paper
Biedermann T, Pontiggia L, Böttcher-Haberzeth S, et al. Human eccrine sweat gland cells can reconstitute a stratified epidermis. J Invest Dermatol. 2010,130(8):1996-2009. doi: 10.1038/jid.2010.83
Read this paper