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ProductsBack/Filaggrin antibody [FLG01]
The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303
The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303
The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303

Filaggrin antibody [FLG01]

GTX23137
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFLG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Filaggrin antibody [FLG01]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Immunohistochemistry (formalin fixed paraffin embedded): use at a concentration of 2 - 4microg / ml for 30 min at RT. Optimal dilutions / concentrations should be determined by the end user. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    FLG01
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2312
  • Target name
    FLG
  • Target description
    filaggrin
  • Target synonyms
    ATOD2; epidermal filaggrin; filaggrin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP20930
  • Protein Name
    Filaggrin
  • Scientific Description
    The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Lithospermum erythrorhizon Alleviates Atopic Dermatitis-like Skin Lesions by Restoring Immune Balance and Skin Barrier Function in 2.4-Dinitrochlorobenzene-Induced NC/Nga Mice. Oh JS et al., 2021 Sep 15, Nutrients
    Read more
  • Orchestrated control of filaggrin-actin scaffolds underpins cornification. Gutowska-Owsiak D et al., 2018 Apr 1, Cell Death Dis
    Read more
  • Eczema in Psoriatico: An Important Differential Diagnosis Between Chronic Allergic Contact Dermatitis and Psoriasis in Palmoplantar Localization. Kolesnik M et al., 2018 Jan 12, Acta Derm Venereol
    Read more
  • A mechanistic target of rapamycin complex 1/2 (mTORC1)/V-Akt murine thymoma viral oncogene homolog 1 (AKT1)/cathepsin H axis controls filaggrin expression and processing in skin, a novel mechanism for skin barrier disruption in patients with atopic dermatitis. Naeem AS et al., 2017 Apr, J Allergy Clin Immunol
    Read more
  • Human eccrine sweat gland cells can reconstitute a stratified epidermis. Biedermann T et al., 2010 Aug, J Invest Dermatol
    Read more

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Figure 1. Western blot analysis of Filaggrin using anti-Filaggrin antibody (PB9870). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filaggrin antigen affinity purified polyclonal antibody (Catalog # PB9870) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filaggrin at approximately 435 kDa. The expected band size for Filaggrin is at 435 kDa.
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