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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA008712HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA008712HA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA008712HA01HU at dilution of 1:100

FLG Antibody

CSB-PA008712HA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFLG
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Overview

  • Supplier
    Cusabio
  • Product Name
    FLG Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2312
  • Target name
    FLG
  • Target description
    filaggrin
  • Target synonyms
    ATOD2; epidermal filaggrin; filaggrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20930
  • Protein Name
    Filaggrin
  • Scientific Description
    Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Filaggrin using anti-Filaggrin antibody (PB9870). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filaggrin antigen affinity purified polyclonal antibody (Catalog # PB9870) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filaggrin at approximately 435 kDa. The expected band size for Filaggrin is at 435 kDa.
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ICC/IF analysis of PFA-fixed HeLa cells using GTX03270 Filaggrin antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:200
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