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ICC/IF analysis of PFA-fixed HeLa cells using GTX03270 Filaggrin antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:200
ICC/IF analysis of PFA-fixed HeLa cells using GTX03270 Filaggrin antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:200
ICC/IF analysis of PFA-fixed HeLa cells using GTX03270 Filaggrin antibody. Red : Primary antibody Green : beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100 Dilution : 1:200

Filaggrin antibody

GTX03270
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFLG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Filaggrin antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2312
  • Target name
    FLG
  • Target description
    filaggrin
  • Target synonyms
    ATOD2; epidermal filaggrin; filaggrin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20930
  • Protein Name
    Filaggrin
  • Scientific Description
    The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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The data was published in the journal J Allergy Clin Immunol in 2017.PMID: 27913303
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Figure 1. Western blot analysis of Filaggrin using anti-Filaggrin antibody (PB9870). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filaggrin antigen affinity purified polyclonal antibody (Catalog # PB9870) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filaggrin at approximately 435 kDa. The expected band size for Filaggrin is at 435 kDa.
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