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FIS1 Antibody (clone 1G9)

LS-C105139
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFIS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FIS1 Antibody (clone 1G9)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Tissue lysate) - positive control human liver. Western Blot (Cell lysate) - positive control A-431. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human small Intestine. Sandwich ELISA (Recombinant protein).. ELISA, WB Western Blot (Tissue lysate) - positive control human liver. Western Blot (Cell lysate) - positive control A-431. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections, 3 ug/ml) - positive control human small Intestine. Sandwich ELISA (Recombinant protein). IHC-P
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G9
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51024
  • Target name
    FIS1
  • Target description
    fission, mitochondrial 1
  • Target synonyms
    CGI-135; FIS1 homolog; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; mitochondrial fission 1 protein; mitochondrial fission molecule; tetratricopeptide repeat domain 11; tetratricopeptide repeat protein 11; TPR repeat protein 11; TTC11
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FIS1 using anti-FIS1 antibody (A01932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: human HEK293 whole cell lysates, Lane 10: human A549 whole cell lysates, Lane 11: human Hela whole cell lysates, Lane 12: human Raji whole cell lysates, Lane 13: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
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