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ProductsBack/[KO Validated] TTC11/FIS1 Monoclonal Antibody

[KO Validated] TTC11/FIS1 Monoclonal Antibody

CAB19666
Assay Genie
TargetFIS1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    [KO Validated] TTC11/FIS1 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IHC,IF,IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID51024
  • Target name
    FIS1
  • Target description
    fission, mitochondrial 1
  • Target synonyms
    CGI-135, TTC11, mitochondrial fission 1 protein, FIS1 homolog, H_NH0132A01.6, TPR repeat protein 11, fission 1 (mitochondrial outer membrane) homolog, hFis1, mitochondrial fission molecule, tetratricopeptide repeat domain 11, tetratricopeptide repeat protein 11
  • Host
    Rabbit
  • Protein IDQ9Y3D6
  • Protein Name
    Mitochondrial fission 1 protein
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FIS1 using anti-FIS1 antibody (A01932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: human HEK293 whole cell lysates, Lane 10: human A549 whole cell lysates, Lane 11: human Hela whole cell lysates, Lane 12: human Raji whole cell lysates, Lane 13: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
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Immunocytochemical staining and detection of endogenous Fis1 (red) (right) and endogenous mitochondrial Hsp70 (green) (left) in methanol fixed HeLa cells using anti-Fis1, pAb (Prod. No. AG-20B-0007V).Picture courtesy of P. Parone, University of G
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