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Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FIS1/TTC11 Polyclonal Antibody, Unconjugated (bs-7646R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FIS1/TTC11 Polyclonal Antibody, Unconjugated (bs-7646R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti FIS1/TTC11 Polyclonal Antibody, Unconjugated (bs-7646R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

FIS1 Polyclonal Antibody

BS-7646R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFIS1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    FIS1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID51024
  • Target name
    FIS1
  • Target description
    fission, mitochondrial 1
  • Target synonyms
    CGI-135; FIS1 homolog; fission 1 (mitochondrial outer membrane) homolog; H_NH0132A01.6; hFis1; mitochondrial fission 1 protein; mitochondrial fission molecule; tetratricopeptide repeat domain 11; tetratricopeptide repeat protein 11; TPR repeat protein 11; TTC11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3D6
  • Protein Name
    Mitochondrial fission 1 protein
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • PM2.5, SO2 and NO2 co-exposure impairs neurobehavior and induces mitochondrial injuries in the mouse brain. Ku T et al., 2016 Nov, Chemosphere
    Read more

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Figure 1. Western blot analysis of FIS1 using anti-FIS1 antibody (A01932-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse spleen tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse NIH-3T3 whole cell lysates, Lane 8: mouse RAW264.7 whole cell lysates, Lane 9: human HEK293 whole cell lysates, Lane 10: human A549 whole cell lysates, Lane 11: human Hela whole cell lysates, Lane 12: human Raji whole cell lysates, Lane 13: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FIS1 antigen affinity purified polyclonal antibody (Catalog # A01932-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FIS1 at approximately 17KD. The expected band size for FIS1 is at 17KD.
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